Journal: BMC Cardiovascular Disorders

Article Title: Loss of ectonucleotidases from the coronary vascular bed after ischemia-reperfusion in isolated rat heart

doi: 10.1186/1471-2261-13-53

Figure Lengend Snippet: Metabolism of ATP (A), ADP (B), AMP (C) and adenosine (Ado, D) during a single pass of coronary circulation. Each substrate at 200 μM (0.3 ml) was added to the coronary perfusate after 20 min stabilization of the normal hearts. Metabolites in the coronary effluents of the first one minute fraction after injection of the substrate were measured by HPLC ( Open columns; initial substrate, black columns; metabolites). Data are shown as percent of total metabolites collected. All values are means ± S.E.M (n = 13).

Article Snippet: The samples containing adenine nucleotide metabolites were analyzed using a JASCO HPLC system equipped with an analytical YMC-Pack ODS-A column (S-5, 4.6 X 100 mm, YMC Inc. Kyoto, Japan) equilibrated at 40°C with 50 mM NaH 2 PO 4 (pH 5.5 adjusted with H 3 PO 4 ) at a flow rate of 1 ml/min [ , ].

Techniques: Injection

Journal: BMC Cardiovascular Disorders

Article Title: Loss of ectonucleotidases from the coronary vascular bed after ischemia-reperfusion in isolated rat heart

doi: 10.1186/1471-2261-13-53

Figure Lengend Snippet: Effect of ischemia and reperfusion on metabolism of eATP (A), eAMP (B) and adenosine (C). After 20 min equilibration, the hearts (n = 5) were subjected to a 30 min-ischemia, followed by reperfusion for 30 min. Each substrate at 10 μM (0.3 ml) was added to the coronary perfusate and subsequent metabolites in the coronary effluents of the first one minute fraction after injection of the substrate were measured by HPLC. Control metabolism was examined in heart after a time-matched control perfusion (80 min, n = 5). Open columns; metabolites from the control hearts, dark columns; metabolites from ischemic heart. Data are shown as percent of total metabolites collected. All values are means ± S.E.M (n = 5). *Significantly different from the control value at P < 0.05 by Student’s t -test.

Article Snippet: The samples containing adenine nucleotide metabolites were analyzed using a JASCO HPLC system equipped with an analytical YMC-Pack ODS-A column (S-5, 4.6 X 100 mm, YMC Inc. Kyoto, Japan) equilibrated at 40°C with 50 mM NaH 2 PO 4 (pH 5.5 adjusted with H 3 PO 4 ) at a flow rate of 1 ml/min [ , ].

Techniques: Injection

Journal: BMC Cardiovascular Disorders

Article Title: Loss of ectonucleotidases from the coronary vascular bed after ischemia-reperfusion in isolated rat heart

doi: 10.1186/1471-2261-13-53

Figure Lengend Snippet: Adenine nucleotide metabolites in the effluents immediately after reperfusion. After 30 min-ischemia, hearts were reperfused with a normal solution, and effluents were collected every 20 sec for 120 sec. Effluents were also collected at pre-ischemia. Endogenous adenine nucleotide metabolites released from hearts into the effluents were measured by HPLC. Data are means ±S.E.M (n = 10). Ado; adenosine, IN; inosine, HX; hypoxanthine.

Article Snippet: The samples containing adenine nucleotide metabolites were analyzed using a JASCO HPLC system equipped with an analytical YMC-Pack ODS-A column (S-5, 4.6 X 100 mm, YMC Inc. Kyoto, Japan) equilibrated at 40°C with 50 mM NaH 2 PO 4 (pH 5.5 adjusted with H 3 PO 4 ) at a flow rate of 1 ml/min [ , ].

Techniques:

Journal: BMC Cardiovascular Disorders

Article Title: Loss of ectonucleotidases from the coronary vascular bed after ischemia-reperfusion in isolated rat heart

doi: 10.1186/1471-2261-13-53

Figure Lengend Snippet: Effects of aging on the changes in ATP hydrolysis after ischemia-reperfusion in the coronary circulation . Control perfusion and ischemia-reperfusion were performed as indicated in Figure in young adult and aged rats. A substrate of eATP (10 μM, 0.3 ml) was administrated into the coronary perfusate and subsequent metabolites in the effluent were measured by HPLC. Data are shown as percent of total metabolites collected from control hearts (A) and ischemia-reperfusion hearts (B) . Gray columns: young adult rats , black columns: aged rats. All values are means ± S.E.M (n = 5). *P < 0.05 , **P < 0.01 significantly different from the control value by Student’s t -test.

Article Snippet: The samples containing adenine nucleotide metabolites were analyzed using a JASCO HPLC system equipped with an analytical YMC-Pack ODS-A column (S-5, 4.6 X 100 mm, YMC Inc. Kyoto, Japan) equilibrated at 40°C with 50 mM NaH 2 PO 4 (pH 5.5 adjusted with H 3 PO 4 ) at a flow rate of 1 ml/min [ , ].

Techniques: